Are Barcodes always on R1 and Sequence data on R2?

No, it really depends on the protocol. In some protocols this convention is swapped, in others the barcodes can be distributed across both reads.

Still have questions?

Want to embed this snippet (FAQ) in your GTN Tutorial?

{% snippet  topics/transcriptomics/tutorials/scrna-umis/faqs/r1r2.md %}