Best practices for loading fastq data into Galaxy


  • As of release 17.09, fastq data will have the datatype fastqsanger auto-detected when that quality score scaling is detected and “autodetect” is used within the Upload tool. Compressed fastq data will be converted to uncompressed in the history.
  • To preserve fastq compression, directly assign the appropriate datatype (eg: fastqsanger.gz).
  • If the data is close to or over 2 GB in size, be sure to use FTP.
  • If the data was already loaded as fastq.gz, don’t worry! Just test the data for correct format (as needed) and assign the metadata type.
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